The hybridoma method is a mainstay for monoclonal antibody (mA😎 production (including the hapten-associated small molecule antibody), during the whole workflow, the accurate and specific screening of positive hybridomas usually determines the success. Commonly, positive antibody-secreting subclones are screened and selected by a solid-phase enzyme-linked immunosorbent assay (ELISA). https://small-molecule-antibody.creative-biolabs.com/screening-for-positive-hybridoma-cells-by-elisa.htm